孙永华

中国科学院研究员

孙永华男，1976年10月生，博士、中国科学院水生生物研究所研究员、博士生导师、鱼类发育与生物技术学科组组长、国家斑马鱼资源中心主任。中国动物学会斑马鱼分会副理事长兼秘书长、中国实验动物学会水生实验动物专业委员会常务委员、湖北省遗传学会理事、湖北省实验动物学会常务理事、中国青年科技工作者协会会员、中国科学院青年联合会委员。入选中国科学院青年创新促进会，获首届国家优秀青年科学基金资助，荣获中国科学院卢嘉锡青年人才奖和首届中国水产青年科技奖等奖项。

研究领域

主要从事鱼类卵质和早期发育的分子调控，以及鱼类基因组操作和定向育种研究(Cell Research, 2000; 中国科学，2014)。

阐明Smad蛋白在早期胚胎中介导BMP信号通路的网络调控机制（Journal of Biological Chemistry, 2014），发现经典Wnt信号通路的新调控因子Lbx2并阐明其激活Wnt信号活性的分子机制(Nature Communications, 2014)；首获转基因鱼的属间克隆鱼，揭示母源因子对克隆鱼发育图式形成的重要作用和克隆鱼败育的分子机制（Biology of Reproduction, 2005; Journal of Physiology, 2014）。建立不依赖于生长激素水平且快速生长的激活型生长激素受体转基因鱼模型（Transgenic Research, 2011, 中国发明专利授权，2013）；利用“合成生物学”概念研制首例具有从头合成omega-6和omega-3不饱和脂肪酸能力的转基因鱼（Marine Biotechnology, 2014，中国发明专利授权，2016）；发现鱼类早期胚胎中广泛存在微同源末端介导的DNA双链断裂修复（MMEJ）机制（Mutation Research, 2015）,创制特异高效的鱼类原始生殖细胞操作技术（Marine Biotechnology, 2013），并将之应用于稳定高效的鱼类基因组操作（中国发明专利授权，2015）。在 Nature Communications、Journal of Biological Chemistry、Mutation Research、Biology of Reproduction、Cell Research、Journal of Physiology、PLoS Genetics、Marine Biotechnology、Transgenic Research以及《中国科学》等国内外学术刊物上发表论文60 余篇。多次作为会议主席或秘书长主持国际性或全国性学术会议，应邀10多次在国际学术会议上作口头报告。主持科技部973 课题、国家自然科学基金优秀青年科学基金、重大研究计划课题和面上项目等一批科研项目。

主持建立国家斑马鱼资源中心，将该中心建成国际学术界公认的全球三大斑马鱼资源库之一。

人物经历

1993－1997：武汉大学生命科学学院本科学习，获学士学位

1997－2002：中国科学院水生生物研究所研究生学习，获博士学位（期间获中国科学院彭荫刚奖学金一等奖及湖北省优秀博士学位论文奖）

2002－2004：中国科学院水生生物研究所助理研究员

2005－2006：法国国家科研中心分子细胞及遗传学研究所（Institut de Génétique et de Biologie Moléculaire et Cellulaire， Centre National de la Recherche Scientifique）博士后及合作访问研究

2006－今：法兰西科学院和中国科学院联合项目“法中科学及应用基金会（La Fondation Franco-Chinoise pour la Science et ses Applications， FFCSA）”博士后研究

2005－2010：中国科学院水生生物研究所副研究员

2010－：中国科学院水生生物研究所研究员，国家斑马鱼资源中心主任

主要代表性论文

1)Liu, L., Pan, L., Li, K., Zhang, Y., Zhu, Z. and Sun, Y.H.* (2016). Zebrafish Health Conditions in the China Zebrafish Resource Center and 20 Major Chinese Zebrafish Laboratories. Zebrafish 13 Suppl 1, S8-S18.

2)He, M.-D., Zhang, F.-H., Wang, H.-L., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2015). Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 780, 86-96.

3)Pang, S.-C., Wang, H.-P., Zhu, Z.-Y. and Sun, Y.-H*. (2015). Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish. Marine Biotechnology, 17(5):593-603.

4)Xie, X., Pan, L. and Sun, Y.* (2015). Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center. Sci China Life Sci 58, 396-399.

5)Ye, D., Zhu, Z. and Sun, Y.* (2015). Fish genome manipulation and directional breeding. Sci China Life Sci 58, 170-177.

6)Wei Z-Q, Xiong F, He M-D, Wang H-P, Zhu Z-Y, Sun Y-H*. (2015) Suppression of Ligase 4 or Xrcc6 activities enhances the DNA homologous recombination efficiency in zebrafish primordial germ cells. Acta Hydrobiol. 39(2):339-48.

7)Wei, C. Y., Wang, H. P., Zhu, Z. Y. and Sun, Y. H.* (2014). Transcriptional factors Smad1 and Smad9 act redundantly to mediate zebrafish ventral specification downstream of smad5.The Journal of Biological Chemistry 289, 6604-6618.

8)Sun, Y. H.* and Zhu, Z. Y. (2014). Cross-species cloning: influence of cytoplasmic factors on development. J Physiol 592, 2375-2379.

9)Lu, F.-I., Sun, Y.-H.*, Wei, C.-Y., Thisse, C. and Thisse, B. (2014). Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway. Nat Commun 5, 5368. (*co-first author)

10)Pang, S. C., Wang, H. P., Li, K. Y., Zhu, Z. Y., Kang, J. X. and Sun, Y. H.* (2014). Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model. Mar Biotechnol (NY) 16, 580-593.

11)Xiong, F., Wei, Z. Q., Zhu, Z. Y. and Sun, Y. H.* (2013). Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems. Mar Biotechnol (NY) 15, 526-539.

12)Liu, C.-W., Xiong, F., Jia, H.-Z., Wang, X.-L., Cheng, H., Sun, Y.-H.*, Zhang, X.-Z., Zhuo, R.-X. and Feng, J. (2013a). Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model. Biomacromolecules 14, 358-366.

13)Wang, Y.-W., Wei, C.-Y., Dai, H.-P., Zhu, Z.-Y. and Sun, Y.-H.* (2013). Subtractive phage display technology identifies zebrafish marcksb that is required for gastrulation. Gene 521, 69-77.

14)Ishtiaq Ahmed, A. S., Xiong, F., Pang, S. C., He, M. D., Waters, M. J., Zhu, Z. Y. andSun, Y. H.* (2011). Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct. Transgenic Res 20, 557-567.

15)Chen, C.-H., Sun, Y.-H.*, Pei, D.-S. and Zhu, Z.-Y. (2009). Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements. Developmental Dynamics 238, 2850-2859.

16)Liu, Y., Bourgeois, C. F., Pang, S., Kudla, M., Dreumont, N., Kister, L., Sun, Y.-H., Stevenin, J. and Elliott, D. J. (2009). The Germ Cell Nuclear Proteins hnRNP G-T and RBMY Activate a Testis-Specific Exon. PLoS Genetics 5 (11): e1000707.

17)Liu, J., Sun, Y. H.*, Wang, Y. W., Wang, N., Pei, D. S., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP. Theriogenology 70, 1525-1535.

18)Pei, D. S., Sun, Y. H.*, Chen, C. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2008). Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos. BMC Dev Biol 8, 29.

19) Pei, D. S., Sun, Y. H.*, Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2007). Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs. Theriogenology 68, 1282-1291.

20)Pei, D. S., Sun, Y. H.*, Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2007). Zebrafish GAPDH can be used as a reference gene for expression analysis in cross-subfamily cloned embryos. Anal Biochem 363, 291-293.

21)Wang, N., Sun, Y. H.*, Liu, J., Wang, Y. P. and Zhu, Z. Y. (2007). Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression. Development Genes and Evolution 217, 299-305.

22)Wang, N., Sun, Y. H.*, Liu, J., Wu, G., Su, J. G., Wang, Y. P. and Zhu, Z. Y. (2007). Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. Journal of Biomedical Science 14, 767-776.

23)Liu, J., Sun, Y. H.*, Wang, N., Wang, Y. P. and Zhu, Z. Y. (2006). Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis. Marine Biotechnology 8, 357-365.

24)Sun, Y. H., Chen, S. P., Wang, Y. P., Hu, W. and Zhu, Z. Y. (2005). Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (Cyprinus carpio) nuclei and goldfish (Carassius auratus) enucleated eggs. Biol Reprod 72, 510-515.

25)Liu, W. Y., Wang, Y., Sun, Y. H.*, Wang, Y. P., Chen, S. P. and Zhu, Z. Y. (2005). Efficient RNA interference in zebrafish embryos using siRNA synthesized with SP6 RNA polymerase. Development Growth & Differentiation 47, 323-331.

26)Wu, B., Sun, Y. H.*, Wang, Y. W., Wang, Y. P. and Zhu, Z. Y. (2005). Characterization of transgene integration pattern in F4 hGH-transgenic common carp (Cyprinus carpio L.). Cell Research 15, 447-454. (* co-first author)

27) Wu, B., Sun, Y. H.*, Wang, Y. P., Wang, Y. W. and Zhu, Z. Y. (2004). Sequences of transgene insertion sites in transgenic F4 common carp. Transgenic Research 13, 95-96. (* co-first author)

28)Wu, G., Sun, Y. H.* and Zhu, Z. Y. (2003). Growth hormone gene transfer in common carp. Aquatic Living Resources 16, 416-420. (* co-first author)

29)Sun, Y., Chen, S., Wang, Y. and Zhu, Z. (2000). The onset of foreign gene transcription in nuclear-transferred embryos of fish. Sci China C Life Sci 43, 597-605.

30)Zhu, Z. Y. and Sun, Y. H. (2000). Embryonic and genetic manipulation in fish. Cell Research 10, 17-27.

31)张峰华, 王厚鹏, 黄思雨, 熊凤, 朱作言,孙永华* (2016). 两种密码子优化的 Cas9 编码基因在斑马鱼胚胎中基因敲除效率的比较. 遗传 38, 144-154.

发明专利目录

1)孙永华，庞少臣，康景轩，朱作言.一种提高鱼类omega-3多不饱和脂肪酸的方法及应用.2016年获中国发明专利授权，专利号：ZL 201410155088.4

2)孙永华，熊凤，魏志强，朱作言.一种定向筛选同源重组PGC的载体及构建方法和应用. 2015年获中国发明专利授权，专利号：ZL 201310341471.4

3)孙永华,朱作言,熊凤, Ishtiaq Ahmed, Mike Waters.一种鱼类持续激活生长激素受体基因及制备方法和用途. 2013年获中国发明专利授权，专利号：ZL 201110141084.7.

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